- Cells to mid log/ need ~ 5OD. Spin down and wash w/ 1mL H2O
- Transfer to 1.5mL round bottom screw cap tube
- add 200 ul SDS sample buffer + (5% BME, 50mM NaF, 1mM PMSF)-add to stock just before use; + 200ul glass beads
- Beat 90sec (setting 9) in cold room and spin down 2", 4oC.
Sample buffer: 4% SDS 5mL+
80mM Tris Cl 6.8
Final 10% glycerol
10mM EDTA
0.2% BFB
CATHYS NO BOIL YEAST EXTRACTS
2010-05-26 22:59:53 来源：不详 评论：0 点击：
Cells to mid log/ need ~ 5OD. Spin down and wash w/ 1mL H2O
Transfer to 1.5mL round bottom screw cap tube
add 200 u
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