微生物发酵培养基的优化方法
2007-10-19 14:45:05   来源:本站原创   评论:0 点击:

微生物的发酵培养基一般由碳源、氮源、水、无机盐、微量元素、生长因子和前体组成,发酵原料往往是成份不完全清楚的物质,这给发酵培养基的优化提出了非常高的难度。在优化的过程中由于因子和因子水平的过多,如果只用一种方法进行试验设计,很难得到理想的结果。我们可以利用

using response surface methodology.J Ind Microbiol Biotechnol.2003,30:682-690

[25]Konya A,Jekkel A,Suto J.Optimization of compactin fermentation.Journal of Industrial Microbiology & Biotechnology.1998,20:150-152

[26]Jatinder K,Chadha B S,Saini H S.Optimization of culture conditions for production of cellulases and xylanases by scytalicium thermophilum using Response Surface Methodology.World Journal of Microbiology & Biotechnology.2005,10:

[27]Sadhukhan AK,Ramana Murthy MV,Ajaya Kumar R.Optimization of mycophenolic acid production in solid state fermentation using response surface methodology.Journal of Industrial Microbiology & Biotechnology.1999,22:33-38

[28]Charin Techapun,Suphawat Sinsuwongwat,Masanori Watanabe.Production of cellulase-free xylanase by a thermotolerant Streptomyces sp.grown on agricultural waste and media optimization using mixture design and Plackett-Burman experimental design methods.Biotechnology Letters.2002,24:1437-1442

Strategies for Optimization Fermentation Medium

Jisan Yu, Kui Hong

(Institute of Tropical Biological Sciences, Chiese Acadeny of Tropical Agricultural Science, Haikou 571101)

Abstract: Microbial fermentation medium include carbon, nitrogen, water, inorganic salt, microelement, growth genes and precursors. There are materials for fermentation complete unknown. These matters cause many difficulties for optimization fermentation medium. There are so many factors in this process, we cann’t get the perfect outcome if just using one technique for the experimentation. Using Plackett-Burman method or Uniform design for experimentations and get linchpin factors which affectting great in product from many factors. Then, optimization linchpin factors with Orthogonal design or Response surface methodology. We can get the better outcome for this strategy.

Key words: fermentation medium,optimization,response surface methodology,Orthogonal design,Plackett-Burman method

相关热词搜索:发酵培养基 优化方法 响应面分析法 正交

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