Cleavage Sites Table for Fusion Proteins：
| PROTEASE |
COMPANY |
Protease Capture |
| Thrombin Leu-Val-Pro-Arg��Gly-Ser |
Amersham-Biosciences, Novagen, SIGMA, Roche |
Benzamidine-Agarose |
| Factor Xa Ile-Glu/Asp-Gly-Arg�� |
Amersham-Biosciences, New England Biolabs, Roche |
Benzamidine-Agarose |
| Enterokinase Asp-Asp-Asp-Asp-Lys�� |
New England Biolabs, Novagen, Roche |
Trypsin Inhibitor-Agarose |
| TEV protease Glu-Asn-Leu-Tyr-Phe-Gln��Gly |
Invitrogen – Life Technologies |
Ni-NTA (6His recomb. TEV) |
| PreScission Leu-Glu-Val-Leu-Phe-Gln��Gly-Pro |
Amersham-Biosciences |
GSTrap for GST fusion enzyme |
| TAGZyme His-tag removal by Exoproteolytic Digestion |
Qiagen |
Ni-NTA (6His recomb. enzyme) |
| Intein Site dithiothreitol cleavage |
New England Biolabs |
DTT elimination by dialysis |
| HRV 3C Protease Leu-Glu-Val-Leu-Phe-Gln��Gly-Pro |
Novagen |
Ni-NTA (6His recomb. enzyme) |
| SUMO Protease recognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMO |
Invitrogen – Life Technologies, LifeSensors |
Ni-NTA (6His recomb. enzyme) |
